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| 25.09.2012 | 20:15 | Royal Mouscron-Péruwelz | - | Standard Liège | 2:3 (1:0, 1:1) n.V. | |
| 26.09.2012 | 20:00 | RAEC Mons | - | RU Wallonne Ciney | 3:0 (1:0) | |
| 20:00 | Sporting Lokeren | - | KFC Dessel Sport | 3:0 (3:0) | ||
| 20:00 | KAA Gent | - | Bocholter VV | 8:0 (4:0) | ||
| 20:00 | R. White Star Woluwe FC | - | KV Kortrijk | 1:2 (1:1, 1:1) n.V. | ||
| 20:00 | RSC Anderlecht | - | RBD Borinage | 2:0 (0:0) | ||
| 20:00 | Club Brugge KV | - | KVW Zaventem | 3:0 (2:0) | ||
| 20:00 | SV Zulte Waregem | - | KVV Coxyde | 1:0 (0:0) | ||
| 20:00 | Union Saint-Gilloise | - | KRC Genk | 0:6 (0:4) | ||
| 20:00 | KVC Westerlo | - | Lierse SK | 2:0 (2:0) | ||
| 20:00 | SK Deinze | - | Sporting Charleroi | 2:4 (1:0, 2:2) n.V. | ||
| 20:00 | KV Oostende | - | Oud-Heverlee Leuven | 2:1 (0:0) | ||
| 20:00 | KV Mechelen | - | Entente Bertrigeoise | 6:0 (1:0) | ||
| 20:00 | FC Brussels | - | Waasland-Beveren | 1:3 (0:0, 1:1) n.V. | ||
| 20:30 | Hoogstraten VV | - | Cercle Brugge | 1:3 (1:2) | ||
| 20:45 | Sint-Truidense VV | - | Beerschot AC | 4:2 (1:0, 1:1, 1:1) i.E. |
