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| 12.10.2016 | 17:00 | 1. SC Znojmo | - | FK Varnsdorf | 2:1 (1:1) | |
| 14.10.2016 | 18:00 | FK Ústí nad Labem | - | MFK Vítkovice | 2:1 (2:0) | |
| 15.10.2016 | 10:15 | FK Baník Sokolov | - | Sigma Olomouc | 1:2 (1:2) | |
| 15:30 | MFK Frýdek-Místek, a.s. | - | FK Fotbal Třinec | 4:1 (3:0) | ||
| 16:00 | 1. SK Prostějov | - | FK Pardubice | 3:0 (2:0) | ||
| 16:00 | FC Sellier & Bellot Vlašim | - | Dynamo České Budějovice | 2:1 (1:0) | ||
| 17:00 | Baník Ostrava | - | Viktoria Žižkov | 1:0 (1:0) | ||
| 18:00 | SFC Opava | - | FK MAS Táborsko | 5:0 (2:0) |
| # | Mannschaft | Sp. | S. | U. | N. | Tore | Dif. | Pk. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Sigma Olomouc | 11 | 8 | 3 | 0 | 18:6 | 12 | 27 |
| 2 |
|
Baník Ostrava | 11 | 7 | 4 | 0 | 21:8 | 13 | 25 |
| 3 |
|
SFC Opava | 11 | 6 | 3 | 2 | 25:14 | 11 | 21 |
| 4 |
|
Dynamo České Budějovice | 11 | 6 | 2 | 3 | 15:10 | 5 | 20 |
| 5 |
|
1. SC Znojmo | 11 | 5 | 3 | 3 | 21:16 | 5 | 18 |
| 6 |
|
FC Sellier & Bellot Vlašim | 11 | 5 | 2 | 4 | 18:15 | 3 | 17 |
| 7 |
|
FK Ústí nad Labem | 11 | 5 | 2 | 4 | 15:14 | 1 | 17 |
| 8 |
|
FK Baník Sokolov | 11 | 4 | 4 | 3 | 15:16 | -1 | 16 |
| 9 |
|
MFK Frýdek-Místek, a.s. | 11 | 4 | 3 | 4 | 21:19 | 2 | 15 |
| 10 |
|
Viktoria Žižkov | 11 | 3 | 5 | 3 | 17:16 | 1 | 14 |
| 11 |
|
FK Varnsdorf | 11 | 3 | 3 | 5 | 12:14 | -2 | 12 |
| 12 |
|
FK MAS Táborsko | 11 | 2 | 5 | 4 | 11:18 | -7 | 11 |
| 13 |
|
FK Fotbal Třinec | 11 | 2 | 3 | 6 | 17:25 | -8 | 9 |
| 14 |
|
FK Pardubice | 11 | 1 | 4 | 6 | 6:19 | -13 | 7 |
| 15 |
|
MFK Vítkovice | 11 | 2 | 0 | 9 | 12:23 | -11 | 6 |
| 16 |
|
1. SK Prostějov | 11 | 1 | 2 | 8 | 8:19 | -11 | 5 |
| Aufstieg | |
| Abstieg |
