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| 22.05.2021 | 15:00 | MFK Chrudim | - | Viktoria Žižkov | 3:5 (1:4) | |
| 15:00 | FC Hradec Králové | - | SK Líšeň | 1:1 (0:1) | ||
| 15:00 | FK Ústí nad Labem | - | FK Dukla Praha | 1:0 (1:0) | ||
| 15:00 | FK Fotbal Třinec | - | FK Blansko | 2:0 (2:0) | ||
| 15:00 | FK MAS Táborsko | - | FC Sellier & Bellot Vlašim | 1:0 (1:0) | ||
| 15:00 | 1. SK Prostějov | - | FK Varnsdorf | 0:0 (0:0) | ||
| 15:00 | Slavoj Vyšehrad | - | Vysočina Jihlava | 2:2 (0:1) |
| # | Mannschaft | Sp. | S. | U. | N. | Tore | Dif. | Pk. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FC Hradec Králové | 25 | 17 | 6 | 2 | 49:20 | 29 | 57 |
| 2 |
|
SK Líšeň | 25 | 13 | 10 | 2 | 42:23 | 19 | 49 |
| 3 |
|
1. SK Prostějov | 25 | 12 | 7 | 6 | 39:33 | 6 | 43 |
| 4 |
|
Viktoria Žižkov | 25 | 12 | 3 | 10 | 40:38 | 2 | 39 |
| 5 |
|
FK Ústí nad Labem | 25 | 11 | 5 | 9 | 23:29 | -6 | 38 |
| 6 |
|
FC Sellier & Bellot Vlašim | 25 | 10 | 6 | 9 | 36:31 | 5 | 36 |
| 7 |
|
FK Fotbal Třinec | 25 | 9 | 5 | 11 | 31:32 | -1 | 32 |
| 8 |
|
Vysočina Jihlava | 25 | 8 | 8 | 9 | 41:43 | -2 | 32 |
| 9 |
|
FK Dukla Praha | 25 | 8 | 7 | 10 | 33:30 | 3 | 31 |
| 10 |
|
MFK Chrudim | 25 | 9 | 4 | 12 | 32:33 | -1 | 31 |
| 11 |
|
FK MAS Táborsko | 25 | 8 | 7 | 10 | 25:26 | -1 | 31 |
| 12 |
|
FK Varnsdorf | 25 | 6 | 12 | 7 | 20:26 | -6 | 30 |
| 13 |
|
FK Blansko | 25 | 6 | 6 | 13 | 28:33 | -5 | 24 |
| 14 |
|
Slavoj Vyšehrad | 25 | 1 | 4 | 20 | 15:57 | -42 | 7 |
| Aufstieg | |
| Abstieg |
