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| 24.05.2023 | 17:00 | FK Dukla Praha | - | 1. SK Prostějov | 4:1 (3:0) | |
| 17:00 | FK Viagem Příbram | - | AC Sparta Praha B | 1:0 (0:0) | ||
| 17:00 | FK Varnsdorf | - | MFK Karviná | 2:2 (2:1) | ||
| 17:00 | FK Třinec | - | SFC Opava | 0:1 (0:0) | ||
| 17:00 | Sigma Olomouc B | - | FK MAS Táborsko | 0:3 (0:1) | ||
| 17:00 | Slavia Praha B | - | SK Líšeň | 2:1 (1:1) | ||
| 17:00 | FC Sellier & Bellot Vlašim | - | MFK Chrudim | 4:0 (1:0) | ||
| 17:00 | MFK Vyškov | - | Vysočina Jihlava | 2:1 (0:1) |
| # | Mannschaft | Sp. | S. | U. | N. | Tore | Dif. | Pk. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
MFK Karviná | 29 | 16 | 5 | 8 | 57:37 | 20 | 53 |
| 2 |
|
MFK Vyškov | 29 | 14 | 9 | 6 | 45:29 | 16 | 51 |
| 3 |
|
FK Viagem Příbram | 29 | 14 | 8 | 7 | 46:30 | 16 | 50 |
| 4 |
|
SK Líšeň | 29 | 13 | 7 | 9 | 37:24 | 13 | 46 |
| 5 |
|
FK Dukla Praha | 29 | 13 | 5 | 11 | 48:44 | 4 | 44 |
| 6 |
|
FK Varnsdorf | 29 | 12 | 7 | 10 | 53:44 | 9 | 43 |
| 7 |
|
FK MAS Táborsko | 29 | 11 | 8 | 10 | 37:41 | -4 | 41 |
| 8 |
|
AC Sparta Praha B | 29 | 11 | 6 | 12 | 32:37 | -5 | 39 |
| 9 |
|
FC Sellier & Bellot Vlašim | 29 | 11 | 5 | 13 | 54:44 | 10 | 38 |
| 10 |
|
MFK Chrudim | 29 | 8 | 14 | 7 | 32:32 | 0 | 38 |
| 11 |
|
Sigma Olomouc B | 29 | 9 | 9 | 11 | 39:46 | -7 | 36 |
| 12 |
|
1. SK Prostějov | 29 | 8 | 9 | 12 | 36:56 | -20 | 33 |
| 13 |
|
Slavia Praha B | 29 | 8 | 8 | 13 | 41:53 | -12 | 32 |
| 14 |
|
SFC Opava | 29 | 7 | 10 | 12 | 24:28 | -4 | 31 |
| 15 |
|
Vysočina Jihlava | 29 | 8 | 7 | 14 | 32:51 | -19 | 31 |
| 16 |
|
FK Třinec | 29 | 6 | 9 | 14 | 30:47 | -17 | 27 |
| Aufstieg | |
| Playoffs | |
| Abstieg |
