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| 17.05.2016 | 17:00 | FK Pardubice | - | Dynamo České Budějovice | 2:0 (1:0) | |
| 17:00 | MFK Frýdek-Místek, a.s. | - | FK Fotbal Třinec | 1:1 (1:1) | ||
| 17:00 | Sigma Olomouc B | - | SFC Opava | 1:1 (1:0) | ||
| 17:00 | FK Varnsdorf | - | Slavoj Vyšehrad | 6:1 (4:0) | ||
| 17:30 | FC Hradec Králové | - | FK Baník Sokolov | 2:0 (2:0) | ||
| 18:00 | FK Ústí nad Labem | - | MFK Karviná | 1:3 (1:1) | ||
| 18:00 | 1. SC Znojmo | - | FC Graffin Vlašim | 0:4 (0:1) |
| # | Mannschaft | Sp. | S. | U. | N. | Tore | Dif. | Pk. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FC Hradec Králové | 27 | 16 | 8 | 3 | 43:15 | 28 | 56 |
| 2 |
|
MFK Karviná | 26 | 15 | 8 | 3 | 45:15 | 30 | 53 |
| 3 |
|
1. SC Znojmo | 26 | 15 | 5 | 6 | 51:30 | 21 | 50 |
| 4 |
|
FK Baník Sokolov | 26 | 10 | 11 | 5 | 29:21 | 8 | 41 |
| 5 |
|
FK Ústí nad Labem | 26 | 8 | 11 | 7 | 37:31 | 6 | 35 |
| 6 |
|
FK Pardubice | 26 | 9 | 8 | 9 | 26:25 | 1 | 35 |
| 7 |
|
FK MAS Táborsko | 26 | 7 | 12 | 7 | 32:32 | 0 | 33 |
| 8 |
|
SFC Opava | 26 | 6 | 14 | 6 | 31:31 | 0 | 32 |
| 9 |
|
FK Varnsdorf | 26 | 7 | 10 | 9 | 32:41 | -9 | 31 |
| 10 |
|
MFK Frýdek-Místek, a.s. | 26 | 7 | 9 | 10 | 26:33 | -7 | 30 |
| 11 |
|
FC Graffin Vlašim | 26 | 7 | 8 | 11 | 28:30 | -2 | 29 |
| 12 |
|
Dynamo České Budějovice | 26 | 5 | 14 | 7 | 36:43 | -7 | 29 |
| 13 |
|
FK Fotbal Třinec | 26 | 6 | 8 | 12 | 29:40 | -11 | 26 |
| 14 |
|
Sigma Olomouc B | 27 | 5 | 5 | 17 | 23:49 | -26 | 20 |
| 15 |
|
Slavoj Vyšehrad | 26 | 5 | 5 | 16 | 18:50 | -32 | 20 |
| Aufstieg | |
| Abstieg |
