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| 22.09.2023 | 16:30 | FK MAS Táborsko | - | FK Příbram | 0:1 (0:0) | |
| 23.09.2023 | 10:15 | MFK Chrudim | - | Viktoria Žižkov | 2:2 (1:1) | |
| 10:30 | 1. SK Prostějov | - | FK Varnsdorf | 1:0 (1:0) | ||
| 14:00 | FK Dukla Praha | - | Hanácká Slávia Kroměříž | 3:1 (2:0) | ||
| 16:30 | FC Sellier & Bellot Vlašim | - | SFC Opava | 1:1 (0:1) | ||
| 17:00 | Zbrojovka Brno | - | MFK Vyškov | 0:1 (0:1) | ||
| 24.09.2023 | 10:15 | Sigma Olomouc B | - | SK Líšeň | 0:1 (0:0) | |
| 10:30 | AC Sparta Praha B | - | Vysočina Jihlava | 2:1 (0:1) |
| # | Mannschaft | Sp. | S. | U. | N. | Tore | Dif. | Pk. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
MFK Vyškov | 10 | 7 | 2 | 1 | 23:12 | 11 | 23 |
| 2 |
|
FK Dukla Praha | 10 | 6 | 2 | 2 | 21:11 | 10 | 20 |
| 3 |
|
Zbrojovka Brno | 10 | 6 | 1 | 3 | 13:9 | 4 | 19 |
| 4 |
|
FC Sellier & Bellot Vlašim | 10 | 4 | 6 | 0 | 15:10 | 5 | 18 |
| 5 |
|
FK MAS Táborsko | 10 | 5 | 2 | 3 | 11:10 | 1 | 17 |
| 6 |
|
SFC Opava | 10 | 4 | 3 | 3 | 11:9 | 2 | 15 |
| 7 |
|
SK Líšeň | 10 | 4 | 3 | 3 | 10:10 | 0 | 15 |
| 8 |
|
Vysočina Jihlava | 10 | 4 | 2 | 4 | 18:14 | 4 | 14 |
| 9 |
|
Viktoria Žižkov | 10 | 3 | 2 | 5 | 18:19 | -1 | 11 |
| 10 |
|
MFK Chrudim | 10 | 2 | 5 | 3 | 18:21 | -3 | 11 |
| 11 |
|
FK Příbram | 10 | 4 | 1 | 5 | 11:15 | -4 | 11 |
| 12 |
|
Sigma Olomouc B | 10 | 3 | 2 | 5 | 12:17 | -5 | 11 |
| 13 |
|
Hanácká Slávia Kroměříž | 10 | 3 | 1 | 6 | 11:15 | -4 | 10 |
| 14 |
|
AC Sparta Praha B | 10 | 2 | 3 | 5 | 13:18 | -5 | 9 |
| 15 |
|
1. SK Prostějov | 10 | 2 | 3 | 5 | 12:20 | -8 | 9 |
| 16 |
|
FK Varnsdorf | 10 | 0 | 4 | 6 | 13:20 | -7 | 4 |
| Aufstieg | |
| Relegation | |
| Abstieg |
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# Punktabzug: FK Viagem Příbram (-2) |
