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| 02.08.2023 | 17:00 | 1. SK Prostějov | - | AC Sparta Praha B | 3:3 (1:2) | |
| 18:00 | Zbrojovka Brno | - | FK Dukla Praha | 0:2 (0:1) | ||
| 18:00 | MFK Chrudim | - | FK MAS Táborsko | 0:3 (0:1) | ||
| 18:00 | Vysočina Jihlava | - | Sigma Olomouc B | 6:3 (3:2) | ||
| 18:00 | FK Viagem Příbram | - | SFC Opava | 1:0 (0:0) | ||
| 18:00 | FK Varnsdorf | - | FC Sellier & Bellot Vlašim | 3:3 (2:1) | ||
| 18:00 | Viktoria Žižkov | - | SK Líšeň | 3:1 (0:1) | ||
| 18:00 | MFK Vyškov | - | Hanácká Slávia Kroměříž | 5:1 (2:0) |
| # | Mannschaft | Sp. | S. | U. | N. | Tore | Dif. | Pk. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
MFK Vyškov | 3 | 3 | 0 | 0 | 11:4 | 7 | 9 |
| 2 |
|
Vysočina Jihlava | 3 | 3 | 0 | 0 | 10:5 | 5 | 9 |
| 3 |
|
FK MAS Táborsko | 3 | 3 | 0 | 0 | 5:0 | 5 | 9 |
| 4 |
|
FK Dukla Praha | 3 | 2 | 0 | 1 | 8:5 | 3 | 6 |
| 5 |
|
FK Viagem Příbram | 3 | 2 | 0 | 1 | 5:4 | 1 | 6 |
| 6 |
|
Zbrojovka Brno | 3 | 1 | 1 | 1 | 3:4 | -1 | 4 |
| 7 |
|
1. SK Prostějov | 3 | 1 | 1 | 1 | 5:7 | -2 | 4 |
| 8 |
|
Viktoria Žižkov | 3 | 1 | 0 | 2 | 6:6 | 0 | 3 |
| 9 |
|
FC Sellier & Bellot Vlašim | 3 | 0 | 3 | 0 | 5:5 | 0 | 3 |
| 10 |
|
SFC Opava | 3 | 1 | 0 | 2 | 2:3 | -1 | 3 |
| 11 |
|
Hanácká Slávia Kroměříž | 3 | 1 | 0 | 2 | 4:6 | -2 | 3 |
| 12 |
|
FK Varnsdorf | 3 | 0 | 2 | 1 | 4:5 | -1 | 2 |
| 13 |
|
MFK Chrudim | 3 | 0 | 2 | 1 | 1:4 | -3 | 2 |
| 14 |
|
SK Líšeň | 3 | 0 | 1 | 2 | 2:5 | -3 | 1 |
| 15 |
|
Sigma Olomouc B | 3 | 0 | 1 | 2 | 5:9 | -4 | 1 |
| 16 |
|
AC Sparta Praha B | 3 | 0 | 1 | 2 | 4:8 | -4 | 1 |
| Aufstieg | |
| Relegation | |
| Abstieg |
