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| 25.05.2024 | 17:00 | FK Dukla Praha | - | AC Sparta Praha B | 6:0 (2:0) | |
| 17:00 | FK Příbram | - | MFK Vyškov | 2:0 (1:0) | ||
| 17:00 | Hanácká Slávia Kroměříž | - | FK MAS Táborsko | 0:2 (0:1) | ||
| 17:00 | SK Líšeň | - | MFK Chrudim | 1:2 (0:0) | ||
| 17:00 | Sigma Olomouc B | - | FK Varnsdorf | 2:1 (0:1) | ||
| 17:00 | SFC Opava | - | Vysočina Jihlava | 4:1 (1:1) | ||
| 17:00 | Viktoria Žižkov | - | 1. SK Prostějov | 0:2 (0:0) | ||
| 17:00 | FC Sellier & Bellot Vlašim | - | Zbrojovka Brno | 2:2 (1:1) |
| # | Mannschaft | Sp. | S. | U. | N. | Tore | Dif. | Pk. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FK Dukla Praha | 30 | 18 | 6 | 6 | 55:29 | 26 | 60 |
| 2 |
|
Sigma Olomouc B | 30 | 18 | 3 | 9 | 49:38 | 11 | 57 |
| 3 |
|
FK MAS Táborsko | 30 | 13 | 10 | 7 | 41:26 | 15 | 49 |
| 4 |
|
MFK Vyškov | 30 | 13 | 8 | 9 | 45:38 | 7 | 47 |
| 5 |
|
MFK Chrudim | 30 | 11 | 9 | 10 | 49:48 | 1 | 42 |
| 6 |
|
SFC Opava | 30 | 11 | 7 | 12 | 36:36 | 0 | 40 |
| 7 |
|
FC Sellier & Bellot Vlašim | 30 | 9 | 13 | 8 | 41:43 | -2 | 40 |
| 8 |
|
Viktoria Žižkov | 30 | 11 | 6 | 13 | 44:51 | -7 | 39 |
| 9 |
|
Zbrojovka Brno | 30 | 11 | 6 | 13 | 41:42 | -1 | 39 |
| 10 |
|
SK Líšeň | 30 | 9 | 12 | 9 | 34:34 | 0 | 39 |
| 11 |
|
AC Sparta Praha B | 30 | 10 | 7 | 13 | 52:58 | -6 | 37 |
| 12 |
|
1. SK Prostějov | 30 | 11 | 4 | 15 | 42:52 | -10 | 37 |
| 13 |
|
FK Varnsdorf | 30 | 8 | 12 | 10 | 51:50 | 1 | 36 |
| 14 |
|
Vysočina Jihlava | 30 | 9 | 8 | 13 | 42:46 | -4 | 35 |
| 15 |
|
FK Příbram | 30 | 11 | 4 | 15 | 32:43 | -11 | 35 |
| 16 |
|
Hanácká Slávia Kroměříž | 30 | 6 | 7 | 17 | 33:53 | -20 | 25 |
| Aufstieg | |
| Relegation | |
| Abstieg |
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# Punktabzug: FK Viagem Příbram (-2) |
