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| 21.03.2009 | 15:00 | Slovan Liberec | - | Bohemians Praha | 2:1 (1:0) | |
| 17:00 | 1. FK Příbram | - | Baník Ostrava | 2:1 (0:0) | ||
| 22.03.2009 | 10:15 | Viktoria Žižkov | - | FK Baumit Jablonec | 0:1 (0:0) | |
| 15:00 | Dynamo České Budějovice | - | Viktoria Plzeň | 1:1 (1:0) | ||
| 15:00 | SK Kladno | - | FK Mladá Boleslav | 0:1 (0:0) | ||
| 16:00 | Slavia Praha | - | Tescoma Zlín | 1:0 (0:0) | ||
| 20:00 | 1. FC Brno | - | Sigma Olomouc | 1:2 (0:1) | ||
| 23.03.2009 | 17:15 | FK Teplice | - | AC Sparta Praha | 0:0 (0:0) |
| » Tabelle | Heim | Auswärts | Hinrunde | Rückrunde | 1. Halbzeit | 2. Halbzeit |
| # | Mannschaft | Sp. | S. | U. | N. | Tore | Dif. | Pk. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Slavia Praha | 21 | 15 | 5 | 1 | 44:14 | 30 | 50 |
| 2 |
|
AC Sparta Praha | 21 | 11 | 5 | 5 | 34:18 | 16 | 38 |
| 3 |
|
Slovan Liberec | 21 | 10 | 6 | 5 | 29:22 | 7 | 36 |
| 4 |
|
FK Mladá Boleslav | 21 | 10 | 6 | 5 | 29:24 | 5 | 36 |
| 5 |
|
Baník Ostrava | 21 | 10 | 4 | 7 | 32:21 | 11 | 34 |
| 6 |
|
FK Baumit Jablonec | 21 | 10 | 4 | 7 | 23:24 | -1 | 34 |
| 7 |
|
FK Teplice | 21 | 9 | 5 | 7 | 24:15 | 9 | 32 |
| 8 |
|
Sigma Olomouc | 21 | 8 | 6 | 7 | 26:29 | -3 | 30 |
| 9 |
|
1. FC Brno | 21 | 7 | 6 | 8 | 24:24 | 0 | 27 |
| 10 |
|
Dynamo České Budějovice | 21 | 4 | 13 | 4 | 18:25 | -7 | 25 |
| 11 |
|
Viktoria Plzeň | 21 | 5 | 9 | 7 | 26:28 | -2 | 24 |
| 12 |
|
1. FK Příbram | 21 | 6 | 5 | 10 | 21:28 | -7 | 23 |
| 13 |
|
Bohemians Praha | 21 | 6 | 4 | 11 | 24:32 | -8 | 22 |
| 14 |
|
SK Kladno | 21 | 5 | 5 | 11 | 14:31 | -17 | 20 |
| 15 |
|
Viktoria Žižkov | 21 | 3 | 5 | 13 | 18:30 | -12 | 14 |
| 16 |
|
Tescoma Zlín | 21 | 2 | 6 | 13 | 12:33 | -21 | 12 |
| Champions League Quali. | |
| Europa League | |
| Abstieg |
