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| 17.03.2017 | 18:00 | Dynamo České Budějovice | - | FK Baník Sokolov | 1:1 (0:0) | |
| 18.03.2017 | 10:15 | FK Pardubice | - | 1. SC Znojmo | 0:1 (0:1) | |
| 14:00 | SFC Opava | - | Baník Ostrava | 0:1 (0:0) | ||
| 15:00 | FC Sellier & Bellot Vlašim | - | FK Ústí nad Labem | 1:1 (0:1) | ||
| 15:00 | MFK Vítkovice | - | FK Fotbal Třinec | 0:0 (0:0) | ||
| 15:00 | 1. SK Prostějov | - | MFK Frýdek-Místek, a.s. | 1:2 (1:2) | ||
| 19.03.2017 | 10:15 | Viktoria Žižkov | - | FK Varnsdorf | 1:0 (1:0) | |
| 14:30 | FK MAS Táborsko | - | Sigma Olomouc | 2:0 (0:0) |
| # | Mannschaft | Sp. | S. | U. | N. | Tore | Dif. | Pk. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Sigma Olomouc | 19 | 13 | 4 | 2 | 37:11 | 26 | 43 |
| 2 |
|
Baník Ostrava | 19 | 11 | 6 | 2 | 30:15 | 15 | 39 |
| 3 |
|
SFC Opava | 19 | 10 | 5 | 4 | 36:20 | 16 | 35 |
| 4 |
|
Dynamo České Budějovice | 19 | 9 | 6 | 4 | 24:14 | 10 | 33 |
| 5 |
|
FC Sellier & Bellot Vlašim | 19 | 9 | 5 | 5 | 33:24 | 9 | 32 |
| 6 |
|
1. SC Znojmo | 19 | 8 | 6 | 5 | 33:27 | 6 | 30 |
| 7 |
|
Viktoria Žižkov | 19 | 7 | 6 | 6 | 28:23 | 5 | 27 |
| 8 |
|
MFK Frýdek-Místek, a.s. | 19 | 7 | 6 | 6 | 35:32 | 3 | 27 |
| 9 |
|
FK Varnsdorf | 19 | 7 | 4 | 8 | 27:25 | 2 | 25 |
| 10 |
|
FK MAS Táborsko | 19 | 6 | 6 | 7 | 23:30 | -7 | 24 |
| 11 |
|
FK Ústí nad Labem | 19 | 7 | 3 | 9 | 20:27 | -7 | 24 |
| 12 |
|
FK Fotbal Třinec | 19 | 5 | 6 | 8 | 26:34 | -8 | 21 |
| 13 |
|
FK Baník Sokolov | 19 | 4 | 7 | 8 | 20:28 | -8 | 19 |
| 14 |
|
FK Pardubice | 19 | 4 | 5 | 10 | 13:27 | -14 | 17 |
| 15 |
|
MFK Vítkovice | 19 | 3 | 2 | 14 | 17:34 | -17 | 11 |
| 16 |
|
1. SK Prostějov | 19 | 2 | 3 | 14 | 15:46 | -31 | 9 |
| Aufstieg | |
| Abstieg |
