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| 05.05.2023 | 17:00 | MFK Vyškov | - | FK Viagem Příbram | 2:2 (2:0) | |
| 18:00 | Vysočina Jihlava | - | FK Třinec | 2:1 (0:0) | ||
| 06.05.2023 | 10:15 | MFK Chrudim | - | Slavia Praha B | 0:0 (0:0) | |
| 17:00 | SK Líšeň | - | SFC Opava | 0:0 (0:0) | ||
| 17:00 | FC Sellier & Bellot Vlašim | - | Sigma Olomouc B | 1:4 (1:1) | ||
| 07.05.2023 | 17:00 | FK MAS Táborsko | - | FK Dukla Praha | 2:1 (0:1) | |
| 17:00 | MFK Karviná | - | 1. SK Prostějov | 5:0 (2:0) | ||
| 08.05.2023 | 11:00 | AC Sparta Praha B | - | FK Varnsdorf | 2:0 (1:0) |
| # | Mannschaft | Sp. | S. | U. | N. | Tore | Dif. | Pk. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
MFK Karviná | 26 | 14 | 4 | 8 | 49:35 | 14 | 46 |
| 2 |
|
SK Líšeň | 26 | 12 | 7 | 7 | 34:20 | 14 | 43 |
| 3 |
|
MFK Vyškov | 26 | 11 | 9 | 6 | 40:28 | 12 | 42 |
| 4 |
|
FK Viagem Příbram | 26 | 11 | 8 | 7 | 41:29 | 12 | 41 |
| 5 |
|
FK Dukla Praha | 26 | 12 | 4 | 10 | 43:39 | 4 | 40 |
| 6 |
|
FK Varnsdorf | 26 | 11 | 6 | 9 | 44:36 | 8 | 39 |
| 7 |
|
AC Sparta Praha B | 26 | 11 | 4 | 11 | 29:33 | -4 | 37 |
| 8 |
|
FC Sellier & Bellot Vlašim | 26 | 10 | 5 | 11 | 49:40 | 9 | 35 |
| 9 |
|
Sigma Olomouc B | 26 | 9 | 8 | 9 | 37:39 | -2 | 35 |
| 10 |
|
FK MAS Táborsko | 26 | 9 | 8 | 9 | 28:35 | -7 | 35 |
| 11 |
|
MFK Chrudim | 26 | 7 | 13 | 6 | 27:24 | 3 | 34 |
| 12 |
|
Vysočina Jihlava | 26 | 8 | 7 | 11 | 28:40 | -12 | 31 |
| 13 |
|
1. SK Prostějov | 26 | 7 | 8 | 11 | 32:50 | -18 | 29 |
| 14 |
|
SFC Opava | 26 | 6 | 9 | 11 | 22:25 | -3 | 27 |
| 15 |
|
Slavia Praha B | 26 | 6 | 8 | 12 | 32:47 | -15 | 26 |
| 16 |
|
FK Třinec | 26 | 6 | 8 | 12 | 28:43 | -15 | 26 |
| Aufstieg | |
| Playoffs | |
| Abstieg |
