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| 31.08.2022 | 16:30 | FK Třinec | - | FC Sellier & Bellot Vlašim | 4:0 (1:0) | |
| 16:30 | AC Sparta Praha B | - | Vysočina Jihlava | 1:3 (1:1) | ||
| 17:30 | FK Dukla Praha | - | MFK Vyškov | 2:0 (2:0) | ||
| 17:30 | FK Varnsdorf | - | FK Viagem Příbram | 1:2 (0:1) | ||
| 17:30 | SK Líšeň | - | Sigma Olomouc B | 4:0 (1:0) | ||
| 17:30 | MFK Karviná | - | FK MAS Táborsko | 0:1 (0:0) | ||
| 17:30 | 1. SK Prostějov | - | MFK Chrudim | 1:1 (0:0) | ||
| 17:30 | SFC Opava | - | Slavia Praha B | 1:1 (1:0) |
| # | Mannschaft | Sp. | S. | U. | N. | Tore | Dif. | Pk. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
SK Líšeň | 6 | 5 | 1 | 0 | 12:2 | 10 | 16 |
| 2 |
|
Vysočina Jihlava | 6 | 4 | 1 | 1 | 12:5 | 7 | 13 |
| 3 |
|
Sigma Olomouc B | 6 | 4 | 1 | 1 | 9:8 | 1 | 13 |
| 4 |
|
MFK Karviná | 6 | 3 | 1 | 2 | 11:8 | 3 | 10 |
| 5 |
|
FK Varnsdorf | 6 | 3 | 1 | 2 | 10:7 | 3 | 10 |
| 6 |
|
MFK Vyškov | 6 | 3 | 1 | 2 | 9:6 | 3 | 10 |
| 7 |
|
FK Třinec | 6 | 3 | 1 | 2 | 11:9 | 2 | 10 |
| 8 |
|
SFC Opava | 6 | 2 | 1 | 3 | 6:6 | 0 | 7 |
| 9 |
|
MFK Chrudim | 6 | 2 | 1 | 3 | 7:8 | -1 | 7 |
| 10 |
|
AC Sparta Praha B | 6 | 2 | 1 | 3 | 9:11 | -2 | 7 |
| 11 |
|
FK Dukla Praha | 6 | 2 | 1 | 3 | 6:8 | -2 | 7 |
|
FK Viagem Příbram | 6 | 1 | 4 | 1 | 6:8 | -2 | 7 | |
| 13 |
|
1. SK Prostějov | 6 | 1 | 3 | 2 | 6:9 | -3 | 6 |
| 14 |
|
Slavia Praha B | 6 | 1 | 2 | 3 | 9:12 | -3 | 5 |
| 15 |
|
FK MAS Táborsko | 6 | 1 | 2 | 3 | 4:9 | -5 | 5 |
| 16 |
|
FC Sellier & Bellot Vlašim | 6 | 0 | 0 | 6 | 4:15 | -11 | 0 |
| Aufstieg | |
| Playoffs | |
| Abstieg |
