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| 30.08.2024 | 18:00 | FC Sellier & Bellot Vlašim | - | Sigma Olomouc B | 3:0 (1:0) | |
| 18:00 | Vysočina Jihlava | - | Viktoria Žižkov | 2:1 (1:1) | ||
| 18:00 | SFC Opava | - | MFK Chrudim | 1:2 (0:0) | ||
| 01.09.2024 | 10:15 | Slavia Praha B | - | 1. SK Prostějov | 2:2 (1:1) | |
| 10:15 | Baník Ostrava B | - | Sk Lisen | 0:2 (0:1) | ||
| 15:00 | AC Sparta Praha B | - | FK MAS Táborsko | 1:2 (1:0) | ||
| 17:30 | FK Varnsdorf | - | MFK Vyškov | 1:2 (0:1) | ||
| 18:00 | Zbrojovka Brno | - | FC Zlín | 1:2 (0:0) |
| # | Mannschaft | Sp. | S. | U. | N. | Tore | Dif. | Pk. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FC Zlín | 8 | 7 | 1 | 0 | 12:3 | 9 | 22 |
| 2 |
|
MFK Chrudim | 8 | 7 | 0 | 1 | 20:5 | 15 | 21 |
| 3 |
|
MFK Vyškov | 8 | 4 | 3 | 1 | 9:5 | 4 | 15 |
| 4 |
|
SFC Opava | 8 | 4 | 2 | 2 | 13:11 | 2 | 14 |
| 5 |
|
Sk Lisen | 8 | 3 | 3 | 2 | 10:9 | 1 | 12 |
| 6 |
|
FC Sellier & Bellot Vlašim | 8 | 2 | 5 | 1 | 13:10 | 3 | 11 |
| 7 |
|
Slavia Praha B | 8 | 3 | 1 | 4 | 14:10 | 4 | 10 |
| 8 |
|
1. SK Prostějov | 8 | 2 | 4 | 2 | 7:10 | -3 | 10 |
| 9 |
|
Viktoria Žižkov | 8 | 2 | 3 | 3 | 11:11 | 0 | 9 |
| 10 |
|
FK Varnsdorf | 8 | 2 | 2 | 4 | 12:12 | 0 | 8 |
| 11 |
|
Sigma Olomouc B | 8 | 2 | 2 | 4 | 9:13 | -4 | 8 |
| 12 |
|
Zbrojovka Brno | 8 | 2 | 2 | 4 | 6:11 | -5 | 8 |
| 13 |
|
FK MAS Táborsko | 8 | 2 | 1 | 5 | 8:11 | -3 | 7 |
| 14 |
|
Baník Ostrava B | 8 | 2 | 1 | 5 | 9:21 | -12 | 7 |
| 15 |
|
AC Sparta Praha B | 8 | 1 | 3 | 4 | 12:15 | -3 | 6 |
| 16 |
|
Vysočina Jihlava | 8 | 1 | 3 | 4 | 8:16 | -8 | 6 |
| Aufstieg | |
| Relegation | |
| Abstieg |
