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| 28.04.2001 | 20:00 | SBV Eindhoven | - | FC Den Bosch | 2:2 (1:2) | |
| 20:00 | Dordrecht '90 | - | Go Ahead Eagles | 4:2 (1:2) | ||
| 20:00 | VVV | - | Telstar | 2:3 (1:3) | ||
| 20:00 | Heracles Almelo | - | SBV Excelsior | 2:2 (0:0) | ||
| 20:00 | BV Veendam | - | MVV | 0:1 (0:1) | ||
| 20:00 | HFC Haarlem | - | BV Emmen | 3:0 (3:0) | ||
| 20:00 | Helmond Sport | - | FC Volendam | 3:0 (1:0) | ||
| 20:00 | TOP Oss | - | SC Cambuur | 1:2 (0:0) | ||
| 29.04.2001 | 14:30 | FC Zwolle | - | ADO Den Haag | 2:0 (1:0) |
| # | Mannschaft | Sp. | S. | U. | N. | Tore | Dif. | Pk. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FC Den Bosch | 30 | 22 | 4 | 4 | 59:20 | 39 | 70 |
| 2 |
|
SBV Excelsior | 30 | 17 | 6 | 7 | 76:59 | 17 | 57 |
| 3 |
|
FC Zwolle | 30 | 15 | 9 | 6 | 64:37 | 27 | 54 |
| 4 |
|
SC Cambuur | 30 | 13 | 9 | 8 | 49:41 | 8 | 48 |
| 5 |
|
FC Volendam | 30 | 14 | 5 | 11 | 47:41 | 6 | 47 |
| 6 |
|
Go Ahead Eagles | 30 | 14 | 4 | 12 | 56:59 | -3 | 46 |
| 7 |
|
Helmond Sport | 30 | 12 | 8 | 10 | 64:44 | 20 | 44 |
| 8 |
|
Telstar | 30 | 12 | 7 | 11 | 39:46 | -7 | 43 |
| 9 |
|
SBV Eindhoven | 30 | 11 | 8 | 11 | 53:51 | 2 | 41 |
| 10 |
|
TOP Oss | 30 | 13 | 2 | 15 | 51:54 | -3 | 41 |
| 11 |
|
Dordrecht '90 | 30 | 9 | 9 | 12 | 54:54 | 0 | 36 |
| 12 |
|
BV Emmen | 30 | 10 | 6 | 14 | 49:57 | -8 | 36 |
| 13 |
|
Heracles Almelo | 30 | 9 | 9 | 12 | 35:48 | -13 | 36 |
| 14 |
|
BV Veendam | 30 | 10 | 5 | 15 | 46:60 | -14 | 35 |
| 15 |
|
MVV | 30 | 10 | 4 | 16 | 37:53 | -16 | 34 |
| 16 |
|
ADO Den Haag | 30 | 8 | 7 | 15 | 45:64 | -19 | 31 |
| 17 |
|
HFC Haarlem | 30 | 7 | 9 | 14 | 40:56 | -16 | 30 |
| 18 |
|
VVV | 30 | 7 | 3 | 20 | 34:54 | -20 | 24 |
