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| 01.04.2000 | 20:00 | FC Groningen | - | NAC Breda | 3:2 (2:1) | |
| 20:00 | Dordrecht '90 | - | BV Emmen | 1:3 (0:2) | ||
| 20:00 | SBV Excelsior | - | ADO Den Haag | 2:0 (2:0) | ||
| 20:00 | Go Ahead Eagles | - | Heracles Almelo | 3:1 (1:1) | ||
| 20:00 | HFC Haarlem | - | TOP Oss | 4:1 (1:0) | ||
| 20:00 | Helmond Sport | - | VVV | 0:0 (0:0) | ||
| 20:00 | RBC Roosendaal | - | BV Veendam | 0:2 (0:1) | ||
| 20:00 | FC Volendam | - | Telstar | 0:3 (0:1) | ||
| 03.04.2000 | 20:00 | SBV Eindhoven | - | FC Zwolle | 2:1 (1:0) |
| # | Mannschaft | Sp. | S. | U. | N. | Tore | Dif. | Pk. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
NAC Breda | 28 | 21 | 1 | 6 | 66:28 | 38 | 64 |
| 2 |
|
FC Zwolle | 28 | 18 | 6 | 4 | 72:34 | 38 | 60 |
| 3 |
|
FC Groningen | 28 | 18 | 5 | 5 | 66:27 | 39 | 59 |
| 4 |
|
BV Emmen | 28 | 15 | 4 | 9 | 39:34 | 5 | 49 |
| 5 |
|
SBV Excelsior | 28 | 15 | 3 | 10 | 57:41 | 16 | 48 |
| 6 |
|
RBC Roosendaal | 28 | 13 | 5 | 10 | 50:40 | 10 | 44 |
| 7 |
|
Heracles Almelo | 28 | 11 | 6 | 11 | 34:35 | -1 | 39 |
| 8 |
|
Dordrecht '90 | 28 | 10 | 7 | 11 | 47:47 | 0 | 37 |
| 9 |
|
SBV Eindhoven | 28 | 11 | 3 | 14 | 32:53 | -21 | 36 |
| 10 |
|
ADO Den Haag | 28 | 10 | 5 | 13 | 27:41 | -14 | 35 |
| 11 |
|
BV Veendam | 28 | 9 | 7 | 12 | 40:51 | -11 | 34 |
| 12 |
|
Go Ahead Eagles | 28 | 9 | 6 | 13 | 48:46 | 2 | 33 |
| 13 |
|
VVV | 28 | 8 | 9 | 11 | 34:49 | -15 | 33 |
| 14 |
|
Telstar | 28 | 10 | 2 | 16 | 35:47 | -12 | 32 |
| 15 |
|
Helmond Sport | 28 | 9 | 2 | 17 | 33:50 | -17 | 29 |
| 16 |
|
HFC Haarlem | 28 | 8 | 4 | 16 | 47:59 | -12 | 28 |
| 17 |
|
TOP Oss | 28 | 8 | 3 | 17 | 31:52 | -21 | 27 |
| 18 |
|
FC Volendam | 28 | 7 | 6 | 15 | 33:57 | -24 | 27 |
